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pcrbio 1-step go rt-pcr kit  (PCR Biosystems Ltd)


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    Structured Review

    PCR Biosystems Ltd pcrbio 1-step go rt-pcr kit
    Pcrbio 1 Step Go Rt Pcr Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcrbio+1+step/PCRBIO+1-Step+Go+RT-PCR+Kit/custom%40b10-53-05%4010%2E3389%2Ffvets%2E2026%2E1754420
    Average 94 stars, based on 54 article reviews
    pcrbio 1-step go rt-pcr kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Specific Detection of SARS-CoV-2 Variants B.1.1.7 (Alpha) and B.1.617.2 (Delta) Using a One-Step Quantitative PCR Assay
    Article Snippet: .. PCR was performed using the PCRBIO 1-step go reverse transcription-PCR (RT-PCR) kit according to the manufacturer’s instructions. ..

    Article Title: Specific Detection of SARS-CoV-2 Variants B.1.1.7 (Alpha) and B.1.617.2 (Delta) Using a One-Step Quantitative PCR Assay.
    Article Snippet: .. PCR was performed using the PCRBIO 1-step go reverse transcription-PCR (RT-PCR) kit according to the manufacturer’s instructions. ..

    Amplification:

    Article Title: Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance
    Article Snippet: .. For RT-qPCRs, total RNAs treated with Turbo DNAse (ThermoFisher) were amplified in a 10 μL reaction with the PCRBIO 1-Step Go RT-PCR Kit (PCR Biosystems Ltd). .. Amplifications were performed on a StepOnePlus machine (Applied Biosystems) under the following cycling conditions: one cycle of retrotranscription at 45 °C for 10 min; one denaturing cycle of 95 °C for 2 min and 40 cycles of 95 °C for 5 s and 60 °C for 30 s; and a melting curve from 60 to 95 °C with 0.3 °C step increases.

    Article Title: Expression of semaphorin-3A in the joint and role in osteoarthritis.
    Article Snippet: .. With an input of 5 μg, RNA was reverse‐transcribed together with cDNA amplification by qPCRs in duplicates using the PCRBIO 1‐Step Go RT‐PCR Kit (PCR Biosystems) and specific intron‐spanning primers (Integrated DNA Technologies [IDT] in a CFX96 Touch Real‐Time PCR Detection System [Biorad]). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance
    Article Snippet: .. For RT-qPCRs, total RNAs treated with Turbo DNAse (ThermoFisher) were amplified in a 10 μL reaction with the PCRBIO 1-Step Go RT-PCR Kit (PCR Biosystems Ltd). .. Amplifications were performed on a StepOnePlus machine (Applied Biosystems) under the following cycling conditions: one cycle of retrotranscription at 45 °C for 10 min; one denaturing cycle of 95 °C for 2 min and 40 cycles of 95 °C for 5 s and 60 °C for 30 s; and a melting curve from 60 to 95 °C with 0.3 °C step increases.

    Article Title: Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance
    Article Snippet: .. RT-qPCRs For RT-qPCRs, total RNAs treated with Turbo DNAse (ThermoFisher) were ampli ed in a 10 µL reaction with the PCRBIO 1-Step Go RT-PCR Kit (PCR Biosystems Ltd). .. Ampli cations were performed on a StepOnePlus machine (Applied Biosystems) under the following cycling conditions: one cycle of retrotranscription at 45°C for 10 minutes; one denaturing cycle of 95°C for 2 minutes and 40 cycles of 95°C for 5 seconds and 60°C for 30 seconds; and a melting curve from 60°C to 95°C with 0.3°C step increases.

    Article Title: Peptidoglycan architecture dictates protein interactions, tissue tropism, and arthritis in the Lyme disease spirochete Borrelia burgdorferi
    Article Snippet: .. All reactions were performed on the same plate, using PCRBIO 1-Step Go RT-PCR Kit (PCR Biosystems) following the recommended procedures. ..

    Reverse Transcription:

    Article Title: Expression of semaphorin-3A in the joint and role in osteoarthritis.
    Article Snippet: .. With an input of 5 μg, RNA was reverse‐transcribed together with cDNA amplification by qPCRs in duplicates using the PCRBIO 1‐Step Go RT‐PCR Kit (PCR Biosystems) and specific intron‐spanning primers (Integrated DNA Technologies [IDT] in a CFX96 Touch Real‐Time PCR Detection System [Biorad]). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Expression of semaphorin-3A in the joint and role in osteoarthritis.
    Article Snippet: .. With an input of 5 μg, RNA was reverse‐transcribed together with cDNA amplification by qPCRs in duplicates using the PCRBIO 1‐Step Go RT‐PCR Kit (PCR Biosystems) and specific intron‐spanning primers (Integrated DNA Technologies [IDT] in a CFX96 Touch Real‐Time PCR Detection System [Biorad]). ..

    Control:

    Article Title: SARS-CoV-2 variants circulating in the Fars province, southern Iran, December 2020-March 2021: A cross-sectional study.
    Article Snippet: .. To make sure that the target failures are probably due to the presence of the ORF1a and/or spike deletions, CDC N1 (nucleocapsid) primer and probe set was used as a control in this multiplexed to detect both the wild‐type and variant viruses.18 The detail of the used primer, probes, and targeted genes are given in Table 1.17 Multiplexed RT‐qPCR was done using the PCRBIO 1‐Step Go RT‐PCR Kit (PCR Biosystems Ltd) with 200 nM of N1 primers, 100 nM of N1 probe, 400 nM of the ORF1a and spike primers, 200 nM of ORF1a and spike probes, and 5 μL of nucleic acid in a total reaction volume of 20 μL. ..

    Variant Assay:

    Article Title: SARS-CoV-2 variants circulating in the Fars province, southern Iran, December 2020-March 2021: A cross-sectional study.
    Article Snippet: .. To make sure that the target failures are probably due to the presence of the ORF1a and/or spike deletions, CDC N1 (nucleocapsid) primer and probe set was used as a control in this multiplexed to detect both the wild‐type and variant viruses.18 The detail of the used primer, probes, and targeted genes are given in Table 1.17 Multiplexed RT‐qPCR was done using the PCRBIO 1‐Step Go RT‐PCR Kit (PCR Biosystems Ltd) with 200 nM of N1 primers, 100 nM of N1 probe, 400 nM of the ORF1a and spike primers, 200 nM of ORF1a and spike probes, and 5 μL of nucleic acid in a total reaction volume of 20 μL. ..



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